General Cell Based Assays

A broad array of cell-based assays designed for use with a variety of live cell experiments and procedures. Ideal for molecular and cell biology research applications.

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6,181 – 6,195 of 6,726 results

6,181

Eurofins Discoverx cAMP Hunter eXpress CNR1 (CB1) CHO-K1 GPCR Assay, 2pl Kit. (95-0071E2CP2M)

The cAMP Hunter CNR1 (CB1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CP 55,940) based activation of CNR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CNR1, a naturally Gi/Go coupled GPCR. Upon activation of CNR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.

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6,182

Revvity Health Sciences Inc HTRF Phospho-NDRG1 (Thr346) Detection Kit, 500 Assay Points

HTRF Phospho-NDRG1 (Thr346) Detection Kit, 500 Assay Points

Pricing & Availability

6,183

Molecular Devices cAMP Fluorescent Assay Kit, 384-Well, Bulk, 60 μL

The CatchPoint cAMP Fluorescent Assay Kit is a high-throughput competitive immunoassay for quantifying 3',5'-cyclic adenosine monophosphate (cAMP) and measuring adenylate cyclase activity in biochemical and cell-based assays. The 384-well bulk format supplies reagents for 20 plates (7,680 assay points) and is optimized for fluorescence intensity detection with long signal stability.

384-well bulk format, sufficient for 20 plates (7,680 wells)
Reaction volume 60 μL per well, 50 μL supported for some HTS systems
Fluorescence intensity detection using Stoplight Red substrate
Minimum detectable concentration 0.1 nM (6 fmol/well), EC50 approximately 3.4 nM
Readings stable from 10 minutes up to 24 hours after substrate addition
Store components at 4°C; kit components stable for six months when stored properly

Pricing & Availability

6,184

Revvity Health Sciences Inc HTRF Human & Mouse Phospho-FOXO1 (Ser256) Detection Kit, 10,000 Assay Points

HTRF Human & Mouse Phospho-FOXO1 (Ser256) Detection Kit, 10,000 Assay Points

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6,185

Revvity Health Sciences Inc AlphaScreen cAMP Detection Kit, 10,000 Assay Points

AlphaScreen cAMP Detection Kit, 10,000 Assay Points

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6,186

Eurofins Discoverx PathHunter Mouse CRHR1 β-Arrestin Cell-Based Assay Kit (CHO-K1)

The PathHunter Mouse CRHR1 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CRHR1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.

Pricing & Availability

6,187

Cell Biolabs Inc NAD+/NADH Assay Kit (Colorimetric), 100 assays

Cell Biolabs’ NAD+/NADH Assay Kit (Colorimetric) is a simple assay that measures NAD+ and NADH present in biological samples such as cell lysates or tissue extracts in a 96-well microtiter plate format. The kit is specific for NAD+, NADH, and their ratio. The kit will not detect NADP+ or NADPH. The total NAD+/NADH concentrations of unknown samples are determined by comparison with a known NAD+ standard. Determination of both NAD+ and NADH requires two separate samples for quantification. NAD+ and NADH do not need to be purified from samples, but rather can be extracted individually with a simple acid or base treatment prior to performing the assay. The kit has a detection sensitivity limit of approximately 4 nM NAD+. Each kit provides sufficient reagents to perform up to 100 assays, including blanks, NAD+ standards and unknown samples.

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6,188

Eurofins Discoverx PathHunter eXpress P2RY6 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0500E2CP19M)

The PathHunter P2RY6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. UTP) based activation of P2RY6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY6 and EA-tagged β-Arrestin-2. Activation of P2RY6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.

Pricing & Availability

6,189

Eurofins Discoverx PathHunter eXpress NMBR Total Internalization Assay, 2pl Kit. (93-0939E3CP5M)

The PathHunter NMBR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of NMBR via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged NMBR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the NMBR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.